Sequence primer design program

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Combinatorial Libraries. New Gene Order Wizard. Sequence Optimisation. Codon Usage Adaptation. Length We recommend that you keep your primer length between 17 and 25 nucleotide-long. Sequence specificity Make sure that there is only one binding site in the genome for your primer. Be sure to choose a primer whose sequence is in your vector. We do not recommend to use degenerate primers. The primer should match the template exactly. Near the 3' end an exact match is essential, especially the last 8 bases.

The Tm of the 5' end should be similar to the Tm of the 3' end. Primer sequence Avoid primers that can form hairpin loops or primer-dimers. All that is required is a target sequence, which is copied and pasted into the program, then you have the option to tweak the design parameters.

The output also includes a suggested annealing temperature to use for each primer pair. Do you have any free PCR primer design software suggestions to add to the list? Leave a comment below with your preferred choice and I will be sure to update the list. Hi Thank You Steven for the great help.

I need to know if any of these softwares can help me design multiplex pcr primer sets I need something to help me analyse Self Complementary structures. Hi Nima Many thanks for your comment. For multiplex primer design tools that are free, your choices are rather limited. I did see a recent one called oli2go. I have never used this, but it does look like it may help you out.

If you find any more, please let me know and I can update the list. Best wishes, Steven. Save my name, email, and website in this browser for the next time I comment.

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